Selective susceptibility of cultured striatal neurons to kainic acid

✍ Scribed by Dr. E. R. Mesco; J. A. Joseph; G. S. Roth

• Book ID: 102912935
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 526 KB
• Volume: 31
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490310216

✦ Synopsis

Abstract

Previous work has shown a selective sensitivity of striatal D~2~‐receptor‐containing neurons (D~2~ cells) to kainic acid (KA). In order to see whether this phenomenon exists in cultured cells, which could provide a very accessible model for subsequent mechanistic studies, we examined striatal cultures grown for up to 30 days and labeled with either [^3^H] spiperone, for D~2~‐receptors, or with [^3^H] ‐SCH23390, for D1 receptors. Analysis of the cells was performed with a digital imaging system (RAS). The cultured cells were examined for ligand binding to receptors, cell size, and susceptibility to KA. The D~2~‐cells showed an increased mortality over D~1~ ‐receptor‐containing neurons (D~1~ cells) in the presence of KA, of a magnitude similar to in vivo loss, with larger D~2~‐cells showing the greatest vulnerability.