✍ Scribed by Antoni Beltran; Rosa M. Marcé; Peter A. G. Cormack; David C. Sherrington; Francesc Borrull
No coin nor oath required. For personal study only.
In this paper we describe, for the first time, a molecularly imprinted polymer (MIP) for the antibiotic amoxicillin (AMX), synthesised by a noncovalent molecular imprinting approach and used to extract AMX selectively from urine samples. The MIP was applied as a molecularly selective sorbent in molecularly imprinted SPE (MISPE) in an off‐line mode, where it showed useful cross‐selectivity for a structurally related antibiotic, cephalexin (CPX). By using a MISPE protocol, the MIP was able to selectively extract both AMX and CFX from 5 mL of water spiked with 10 mg/L with recoveries of 75 and 78% for AMX and CFX, respectively. When applied to real samples (urine) at clinically relevant concentrations, recoveries from 2 mL of human urine spiked with 20 mg/L decreased slightly to 65 and 63% for AMX and CFX, respectively. To demonstrate further the selectivity of the MIP obtained, a comparison with commercially available SPE cartridges was performed. Improvements in the retention of both AMX and CFX on the MIP were obtained relative to the commercially available cartridges, and the MISPE extracts were considerably cleaner, due to molecularly selective analyte binding by the MIP.
📜 SIMILAR VOLUMES
## Abstract In the present paper, we describe the synthesis of a cephalexin (CFX) molecularly imprinted polymer (MIP), the direct application of the MIP to SPE for the determination of CFX (which is a β‐lactam antibiotic) in human urine and the use of the MIP in a tandem SPE system to determine CFX
## Abstract A method for molecularly imprinted SPE of the melamine from environmental water samples was investigated. Cyromazine‐imprinted polymers were synthesized in water–methanol systems for the selective extraction of melamine from aqueous samples, followed by HPLC analysis. Molecular recognit
## Abstract A new molecularly imprinted polymer for extraction of crocin from saffron stigmas was prepared using gentiobiose (a glycoside moiety in crocin structure) as a template. Crocin binding to gentiobiose imprinted polymer (Gent‐MIP) was studied in comparison with a blank nonimprinted polymer
## Abstract A molecularly imprinted polymer (MIP) is synthesised by a noncovalent protocol in which ibuprofen was used as a template molecule. The polymer was evaluated chromatographically and it was seen that the MIP showed cross‐reactivity. Subsequently, when this polymer was used as sorbent in S