𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Selective sensitivity of solid tumors to suramin in primary cultures of tumor cells from patients

✍ Scribed by Katalin Csoka; Peter Nygren; Wilhelm Graf; Lars Påhlman; Bengt Glimelius; Rolf Larsson

Publisher
John Wiley and Sons
Year
1995
Tongue
French
Weight
571 KB
Volume
63
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

The antineoplastic activity of suramin is currently the subject of clinical trials. We therefore used the semi-automated fluorometric microculture cytotoxicity assay (FMCA) to evaluate the cytotoxicity of suramin in vitro in primary cultures of cells from patients with hematological or solid tumors. The activity patterns of some standard cytotoxic agents were included for comparison. A total of I59 samples were tested using continuous drug exposure. Surarnin showed relatively high activity against solid tumors, with colorectal, adrenal and kidney carcinomas being the most sensitive, whereas hematological malignancies were more resistant. Surarnin and standard drugs showed very low cross-resistance. The results indicate that suramin is differentially active against some solid tumors with comparatively little activity against hematological tumors. The study provides an experimental motive for evaluation of suramin therapy in patients with solid tumors and exploration of less toxic suramin analogues.

📜 SIMILAR VOLUMES

Detection of tumor-specific cytotoxic dr
Detection of tumor-specific cytotoxic drug activity IN VITRO using the fluorometric microculture cytotoxicity assay and primary cultures of tumor cells from patients
✍ Peter Nygren; Helena Fridborg; Katalin Csoka; Christer Sundström; Manuel De La T 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 French ⚖ 904 KB

The semi-automated fluorometric microculture cytotoxicity assay (FMCA), based on the measurement of fluorescence generated from cellular hydrolysis of fluorescein diacetate (FDA) by viable cells, was employed for cytotoxic drug sensitivity testing of tumor cells from patients with hematological or s

Role of receptor occupancy in the transi
Role of receptor occupancy in the transition from responsive to unresponsive states in cultured breast tumor cells
✍ Philippa D. Darbre; Roger J. B. King 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 387 KB 👁 1 views

Progression from a steroid sensitive to insensitive state is characteristic of breast tumors, but little is known about the molecular mechanisms involved. Changes in steroid receptor can be associated with the progression. This paper reviews the cell culture data pertaining to loss of response and c

Differences in the recognition of tumor-
Differences in the recognition of tumor-specific CD8+ T cells derived from solid tumor, metastatic lymph nodes and ascites in patients with gastric cancer
✍ Koji Kono; Fumiko Ichihara; Hidehiko Iizuka; Takayoshi Sekikawa; and Yoshirou Ma 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 43 KB 👁 2 views

We established gastric cancer-specific CD8 1 T-cell (T CD8 1) lines derived from different lymphocyte sources in the same patients by repeated stimulation with mitomycin-C-treated autologous tumor cells with low-dose interleukin-2, and we compared recognition patterns among the T CD8 1 derived from

Establishment of tumor cell lines from a
Establishment of tumor cell lines from a patient with head and neck cancer and their different sensitivities to anti-cancer agents
✍ Sohtaro Komiyama; Katsuko Matsui; Shohji Kudoh; Ichiro Nogae; Yuichiro Kuratomi; 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 660 KB

The authors established five cell lines from a human head and neck tumor. The five cell lines (HC-2, HC-3, HC-4, HC-7, and HC-9) exhibited different sensitivities to Adriamycin, cisplatin, bleomycin, 5- fluorouracil, vincristine, and daunomycin. The Dw was 200 ng/ml Adriamycin (doxorubicin) for HC-7