Selective recognition of thymidine homopolymer (poly T) oligonucleotide with cobalt(II)–4-[(5-chloro-2-pyridyl)azo]-1,3-diaminobenzene complex
✍ Scribed by Feng Ling Guo; Yuan Fang Li; Cheng Zhi Huang
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 278 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1522-7235
- DOI
- 10.1002/bio.1081
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✦ Synopsis
Abstract
The interactions of cobalt(II)–4‐[(5‐chloro‐2‐pyridyl)azo]‐1,3‐diaminobenzene (5‐Cl‐PADAB) complex with different kinds of homopolymer oligonucleotides in basic medium were investigated based on the measurements of resonance light scattering, UV–vis, circular dichroism spectra and dark field light‐scattering imaging. Experiments showed that only thymidine homopolymer (poly T) oligonucleotides with the length in the range of poly T~6~ to poly T~18~ could interact with the Co(II)–5‐Cl‐PADAB complex in alkaline conditions and cause evident color and spectral change. Thus, the binary complex of Co(II)–5‐Cl‐PADAB could be employed as a visual probe for selectively recognizing the poly T oligonucleotides. Copyright © 2009 John Wiley & Sons, Ltd.