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Selective quenching of RNA in aqueous solution: Association of water to polar groups

✍ Scribed by Povel N. Paus

Book ID
102627681
Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
214 KB
Volume
31
Category
Article
ISSN
0003-2697

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✦ Synopsis

A gradual decline in count rate is observed in liquid scintillation counting of labeled RNA in aqueous solution. This "progressive" quenching is probably caused by selective association of water to the RNA molecules.

MATERIALS AND METHODS

RNA

Labeled RNA was extracted by the method of Parish and Kirby (1) and dissolved in 0.1 M sodium acetate, pH 6, prior to use.

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