Selective quantification of free 3-nitrotyrosine in exhaled breath condensate in asthma using gas chromatography/tandem mass spectrometry
✍ Scribed by Mona Lärstad; Ann-Sofi Söderling; Kenneth Caidahl; Anna-Carin Olin
- Book ID
- 116789963
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 512 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1089-8603
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## Halogenation and nitration of biomolecules have been proposed as key mechanisms of host defense against bacteria, fungi, and viruses. Reactive oxidants also have the potential to damage host tissue, and they have been implicated in disease. In the current studies, we describe specific, sensitiv
A fully validated gas chromatographic-tandem mass spectrometric (GC-tandem MS) method for the accurate and precise quantification of free 3-nitrotyrosine in human plasma at the basal state is described. In the plasma of 11 healthy humans a mean concentration of 2.8 nM (range 1.4 -4.2 nM) for free 3-