Selective procedure for isolation of microorganisms producing pullulanase and isoamylase

✍ Scribed by Rubén D. González; Elvira C. Arenas; Elisa B. Vilches; Miguel A. Billerbeck

• Publisher: Springer-Verlag
• Year: 1993
• Tongue: English
• Weight: 323 KB
• Volume: 7
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00155475

✦ Synopsis

Selective isolation of microorganisms producing pullulanase and isoamylase was accomplished using a two plate detection assay which distinguished both activities and excluded microorganisms producing other extracellular amylases.

Over 115,000 colonies tested, 190 strains producing pullulanase and 57 strains producing isoamylase were isolated; extracellular activities of isolated strains were 0.2 to 4.2 and 0.5 to 2.1 nkat/ml of culture, respectively.