Selective observation of biologically important 15N-labeled metabolites in isolated rat brain and liver by 1H-detected multiple-quantum-coherence spectroscopy

Selective, in Vivo Observation of (5-15N

Selective, in Vivo Observation of (5-15N)Glutamine Amide Protons in Rat Brain by 1H-15N Heteronuclear Multiple-Quantum-Coherence Transfer NMR

✍ K. Kanamori; B.D. Ross; J. Tropp 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 682 KB

The amide protons of [5-15N]glutamine were selectively observed in vivo in the brains of anesthetized, spontaneously breathing rats after intravenous 15NH4+ infusion by 1H-15N heteronuclear multiple-quantum-coherence (HMQC) transfer NMR at 200 MHz for 1H. The peak intensity of the upfield amide prot