Selective modification of cell surface proteins and thymidine transport in hamster cells exposed to cholera toxin
✍ Scribed by Rieber, M. ;Bacalao, J.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1976
- Tongue
- English
- Weight
- 301 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0091-7419
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✦ Synopsis
Abstract
The increased adherence and morphological response which occurs in Chinese hamster ovary cells as a result of exposure to cholera toxin is paralleled by modification in the relative exposure of outer proteins. Mild proteolysis treatment of the cells prelabeled with [^3^H] glucosamine reveals a markedly different kinetics of release of external glycopeptides as a result of exposure to cholera toxin. Selective alterations in external tyrosyl‐rich proteins can also be detected by lactoperoxidase‐catalyzed radioiodination. The above modifications are accompanied by a decrease in the rate of thymidine uptake by toxintreated cells.