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Selective mechanism of action of tebufenozide on lepidopteran cell lines

✍ Scribed by Sundaram, Meenakshi; Palli, Subba R; Krell, P J; Sohi, Sardar S; Retnakaran, Arthur

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
64 KB
Volume
55
Category
Article
ISSN
1526-498X

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✦ Synopsis

The non-steroidal ecdysone agonist, tebufenozide (RH-5992), induces a precocious incomplete molt primarily on lepidopteran insects, but has little or no effect on insects of other orders. 20 Hydroxyecdysone at 10 À7 M induced the transcription factor CHR3 mRNA in CF-203 cells and DHR3 mRNA in DM-2 cells. Tebufenozide even at 10 À10 M induced CHR3 mRNA in lepidopteran CF-203 cells, but even at 10 À5 M it induced only trace levels of DHR3 mRNA in dipteran DM-2 cells. Studies using radiolabelled RH-5992 revealed that lepidopteran cell lines (CF-203 and MD-66) retained more of this compound within the cells than dipteran cell lines (DM-2 and K c ). The ef¯ux of radiolabelled RH-5992 from DM-2 cells was temperature-dependent and was blocked by 10 À5 M ouabain, an inhibitor of Na , K -ATPase, suggesting that the ef¯ux was due to active transport.

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