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Selective isolation of methionine-containing peptides by hydrophobicity modulation

✍ Scribed by Tatsuru Sasagawa; Koiti Titani; Kenneth A. Walsh

Book ID
102626644
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
441 KB
Volume
128
Category
Article
ISSN
0003-2697

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✦ Synopsis

Methionine-containing peptides are selectively isolated from complex digests by taking advantage of successive changes in hydrophobicity and chromatographic mobility induced by S methylation and demethylation of methionyl residues. The peptides are first separated by highperformance liquid chromatography in their S-['4C]methyl forms and then demethylated to facilitate their separation from contaminants during subsequent rechromatography.

πŸ“œ SIMILAR VOLUMES

Selective isolation of tryptophan-contai
Selective isolation of tryptophan-containing peptides by hydrophobicity modulation
✍ Tatsuru Sasagawa; Koiti Titani; Kenneth A. Walsh πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 457 KB

Tryptophan-containing peptides are selectively isolated from complex digests by taking advantage of changes in hydrophobicity and chromatographic mobility induced by reaction with o-nitrophenylsulfenyl chloride. The peptides are first located in crude fractions by monitoring the fluorescence during