𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Selective inhibition of interleukin-1β gene expression in activated RAW 264.7 macrophages by interferon-γ

✍ Scribed by Chujor S. N. Chujor; Lilian Klein; Charles Lam

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
727 KB
Volume
26
Category
Article
ISSN
0014-2980

No coin nor oath required. For personal study only.

✦ Synopsis

Selective inhibition of interleukin-lp gene expression in activated RAW 264.7 macrophages by interferon-y

The ability of interleukin-1 (IL-1) to activate epidermal cell populations supports its role as a key cytokine in the pathogenesis of a number of inflammatory skin diseases. In the present study, we have examined the effect of interferon (1FN)-y on the expression of the IL-lp gene in mouse RAW 264.7 macrophages activated by lipopolysaccharide (LPS) plus tumor necrosis factor (TNF)-a. Incubation of macrophages with both LPS and TNF-a resulted in the expression of both IL-1p and inducible nitric oxide synthase (iNOS) mRNA transcripts and increased the release of IL-1p protein and nitrite production in culture supernatants. Addition of IFN-y up-regulated the expression of the iNOS gene in cells activated by LPS + TNF-a, but significantly suppressed the induction of IL-1p gene expression in a dose-dependent manner. The suppression required neither de novo protein synthesis nor involved destabilization of the mRNA transcripts. Together, these findings suggest that IFN-y can be an important regulatory cytokine in a chronic inflammatory site and may explain its purported antiinflammatory effects in certain dermatological diseases.

📜 SIMILAR VOLUMES

Inhibition of nitric oxide synthase inhi
Inhibition of nitric oxide synthase inhibitors and lipopolysaccharide induced inducible NOS and cyclooxygenase-2 gene expressions by rutin, quercetin, and quercetin pentaacetate in RAW 264.7 macrophages
✍ Yen-Chou Chen; Shing-Chuan Shen; Woan-Ruoh Lee; Wen-Chi Hou; Ling-Ling Yang; Ton 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 220 KB

## Abstract Several natural flavonoids have been demonstrated to perform some beneficial biological activities, however, higher‐effective concentrations and poor‐absorptive efficacy in body of flavonoids blocked their practical applications. In the present study, we provided evidences to demonstrat

Dioscorealide B suppresses LPS-induced n
Dioscorealide B suppresses LPS-induced nitric oxide production and inflammatory cytokine expression in RAW 264.7 macrophages: The inhibition of NF-κB and ERK1/2 activation
✍ Poonsit Hiransai; Suvina Ratanachaiyavong; Arunporn Itharat; Potchanapond Graidi 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 351 KB

## Abstract Dioscorealide B (DB), a naphthofuranoxepin has been purified from an ethanolic extract of the rhizome of __Dioscorea membranacea__ Pierre ex Prain & Burkill which has been used to treat inflammation and cancer in Thai Traditional Medicine. Previously, DB has been reported to have anti‐i