Selective inhibition by monosaccharides of tumor cell cytotoxicity mediated by mouse macrophages, macrophage-like cell lines, and natural killer cells

✍ Scribed by Michael J. Brunda; Robert H. Wiltrout; Howard T. Holden; Luigi Varesio

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: French
• Weight: 834 KB
• Volume: 31
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910310319

✦ Synopsis

Abstract

A series of monosaccharides were tested for their ability to inhibit the effector phase of macrophage‐mediated cytolysis against two susceptible murine tumor target cells, L5I78Y and RL♂I. Two monosaccharides, D‐mannose and N‐acetyl‐D‐galactosamine, were found to decrease cytotoxicity consistently in a dose‐dependent manner. However, D‐mannose preferentially inhibited lysis of RL♂I target cells with little effect on lysis of L5I78Y target cells, while the reverse was found with N‐acetyl‐D‐galactosamine. Neither monosaccharide interfered with the activation of macrophages by polyinosinic:polycytidylic acid. Natural killer cell activity was decreased by a 25 mM concentration of D‐mannose but not by N‐acetyl‐D‐galactosamine, although increasing concentrations of N‐acetyl‐D‐galactosamine were inhibitory. Neither monosaccharide affected cytotoxicity by alloimmune T cells. Cytotoxicity of macrophage‐like cell lines against tumor target cells was also decreased by monosaccharides but the pattern of inhibition was different from that seen with activated macrophage effector cells. Both D‐mannose and N‐acetyl‐D‐galactosamine inhibited glucose oxidation by activated macrophages but only D‐mannose significantly decreased protein synthesis of activated macrophages. These results indicate that monosaccharides can inhibit macrophage‐mediated cytotoxicity in a selective manner with the pattern dependent on the tumor target cell used in the assay.