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Selective induction of mitogen-activated protein kinases in human lens epithelial cells by ultraviolet radiation

✍ Scribed by Joshua A. Bomser

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
338 KB
Volume
16
Category
Article
ISSN
1095-6670

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✦ Synopsis

Abstract

The present study investigates the effects of ultraviolet radiation (UVR) on mitogen‐activated protein kinase (MAPK) activity in human lens epithelial (HLE) cells. Irradiation of HLE cells with ultraviolet B and ultraviolet C radiation activates the stress‐response MAPK proteins, p38 and c‐Jun NH~2~‐terminal kinase (JNK), in a dose‐ and time‐dependent manner, while the extracellular‐regulated signal kinase (ERK 44/42) cascade was not altered by UVR exposure. Ultraviolet A radiation failed to elicit a MAPK response. UVR‐induced MAPK activation does not require protein kinase C or phosphatidylinositol 3‐kinase activity, suggesting that this is not a receptor‐mediated event. Inhibition of ribosomal translation completely abolished UVR‐induced MAPK activation, while treatment with the antioxidant, N‐acetyl cysteine, and mild heat shock had no effect on this activation. These data demonstrate for the first time the selective activation of MAPK cascades in a lens epithelial cell line. © 2002 Wiley Periodicals, Inc. J Biochem Mol Toxicol 16:33–40, 2002; DOI 10.1002/jbt.10016

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