Selective increase of c-myc mRNA levels by methylglyoxal-bis (guanylhydrazone) and novobiocin in serum-stimulated fibroblasts
✍ Scribed by Patricio Aller; Renato Baserga
- Book ID
- 102882610
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 756 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
We have studied the effect of methylglyoxal-bis (guanylhydrazone) (MGBG) and novobiocin on the accumulation of specific mRNAs in serum-stimulated ts13 cells (a temperature-sensitive mutant of the BHK cell line). The RNAs studied included: c-myc, v-ras, ornithine decarboxylase, P-actin, histone H3, and those represented by clones p2F1 and plB6 (Hirschhorn et al., Proc. Natl, Acad. Sci. USA, 815004, 1984) All these RNAs accumulated at higher levels when quiescent cells were serum stimulated for 16 h. Both MCBG (25 pM and 100 pM) and novobiocin (200 pg/ml) effectively prevented the transition from Go to S phase. We found that 100 pM MGBG induced an overaccumulation of c-myc RNA while H 3 RNA was decreased, and the steady-state levels of all other RNAs were the same as in cells stimulated without the drug. Novobiocin prevented the serum-induced increase in the amount of all RNAs, which remained at the same levels as in quiescent cells, with the exception of cmyc, which again accumulated at a higher level in drug-treated cells than in serum-stimulated untreated cells. The possible significance of these results is discussed.