Selective Imaging of Biofilms in Porous Media by NMR Relaxation

✍ Scribed by Brian C. Hoskins; Liv Fevang; Paul D. Majors; Mukul M. Sharma; George Georgiou

• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 354 KB
• Volume: 139
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.1999.1747

✦ Synopsis

Nuclear magnetic resonance imaging (NMRI) techniques were employed to identify and selectively image biological films (biofilm) growing in aqueous systems. Biofilms are shown to affect both the longitudinal (T 1 ) and transverse (T 2 ) NMR relaxation time values of proximal water hydrogens. Results are shown for biofilm growth experiments performed in a transparent parallelplate reactor. A comparison of biofilm distributions by both NMR and optical imaging yielded general agreement for both an openflow system and an idealized porous system (the reactor without and with packed glass beads, respectively). The selective imaging of biofilm by relaxation NMRI is dependent upon the resolution of relaxation times for the fluid phases, dynamic range, and signalto-noise ratio. For open-flow systems, the use of a rapid and quantitative T 2 -sorted NMRI technique was preferred. For porous systems where T 2 values are generally more similar, a T 1 -weighted technique was preferred.