✦ LIBER ✦
Selective Identification of Cooperatively Binding Fragments in a High-Throughput Ligation Assay Enables Development of a Picomolar Caspase-3 Inhibitor
✍ Scribed by Marco F. Schmidt; Adeeb El-Dahshan; Sandro Keller; Jörg Rademann
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 503 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0044-8249
No coin nor oath required. For personal study only.
✦ Synopsis
ure 3 b) by using Equations ( 1) and ( 2) (for all equations, see the Supporting Information). Binding data for various concentrations of 8 were then fitted with the aid of a set of four equilibrium equations [Eqs. ( 3)-( 6)] and three equations of mass conservation [Eqs. ( 7)-( 9)] in order to obtain the ligation constant, K C .