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Selective enrichment and characterization of high affinity ligands from collections of random peptides on filamentous phage

โœ Scribed by Ronald W. Barrett; Steven E. Cwirla; Martha S. Ackerman; Ann M. Olson; Elizabeth A. Peters; William J. Dower


Book ID
107719040
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
918 KB
Volume
204
Category
Article
ISSN
0003-2697

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Selection of high affinity ligands to he
โœ Kok Lian Ho; Khatijah Yusoff; Heng Fong Seow; Wen Siang Tan ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 179 KB

M13 phages that display random disulfide constrained heptapeptides on their gpIII proteins were used to select for high affinity ligands to hepatitis B core antigen (HBcAg). Phages bearing the amino acid sequences C-WSFFSNI-C and C-WPFWGPW-C were isolated, and a binding assay in solution showed that