✦ LIBER ✦
Selective enhancement of metastatic capacity in mouse bladder carcinoma cells after transfection with dna from liver metastases of human colon carcinoma
✍ Scribed by Adriana Radler-Pohl; Jens Pohl Volker Schirrmacher
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- French
- Weight
- 765 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
To identify sequences associated with a metastatic phenotype, D N A fragments isolated from 2 separate human colon carcinoma metastases were transfected into a mouse bladder carcinoma cell line together with the neoR gene as selectable marker. It was found that bulk populations of neomycin resistant cells carrying these human sequences caused more metastases in syngeneic mice than did control cells transfected with calf thymus DNA. Cells isolated from metastases retained the highly metastatic phenotype when transferred to secondary hosts.