Selective depolymerisation of dermatan sulfate: Production of radiolabelled substrates for α-l-iduronidase, sulfoiduronate sulfatase, and β-d-glucuronidase

Selective depolymerisation of keratan su

Selective depolymerisation of keratan sulfate: production of radiolabelled substrates for 6-O-sulfogalactose sulfatase and β-d-galactosidase

✍ John J. Hopwood; Helen Elliott 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 734 KB

Keratan sulfate (KS) was N-deacetylated with anhydrous hydrazine and then degraded with nitrous acid, and the products were reduced with NaBT4. Radiolabelled disaccharides constituted at least 76% of the total oligosaccharide fraction. Three major disaccharides were isolated and identified. Of the t