Selective degradation of β- and γ-cyclodextrin by co-digestion using a CGTase fromBacillus ohbensisand α-glucosidase for efficient α-cyclodextrin recovery
✍ Scribed by Michikatsu Sato; Machiko Watanabe; Hiroto Nagano; Yoshiaki Yagi
- Book ID
- 104636385
- Publisher
- Springer Netherlands
- Year
- 1994
- Tongue
- English
- Weight
- 385 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
A simple and specific recovery method for a-cyclodextrin (a-CD) was developed by employing co-digestion of CD reaction mixtures with CGTase from Bacillus ohbensis and a-glucosidase. The combination of CGTase from B. ohbensis and a-glucosidase, such as a-amylase, j3-amylase, or glucoamylase was examined for the selective degradation of pand y-CD in the CD reaction mixture formed by CGTase from B. macerans. The codigestion of the CD mixture with Taka-amylase and the CGTase resulted in CC-CD and maltodextrins, the combination with P-amylase resulted in a-CD and maltose, and that with glucoamylase resulted in a-CD and glucose. The conditions of selective degradation of p-and y-CD by co-digestion with the CGTase and glucoamylase were optimized as follows: the incubation pH, 5.5; incubation temperature, 50°C; CGTase concentration, 15 u/g of substrate; glucoamylase, 10 u/g of substrate; substrate concentration, 10% (w/v); the incubation time was fixed for 18 hr from the stand point of operation convenience.