✦ LIBER ✦
Selective cleavage of the O6-diphenylcarbamoyl group from sugar-modified guanosines for incorporation into oligo-RNA
✍ Scribed by András Földesi; Anna Trifonova; Zoltán Dinya; Jyoti Chattopadhyaya
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- French
- Weight
- 157 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
✦ Synopsis
A facile conversion of 2',3',5'-O-tri-(Bz, Tol or Ac)-N2-(Ac or iBu)-O6-DPC-guanosine to N2-(Ac or iBu)guanosine has been achieved in 89-98% yield by short treatment with 90% aqueous TFA for smooth cleavage of the DPC group followed by treatment with sodium ethoxide in pyridine-ethanol mixture at room temperature.