Selective cleavage of protonated penetratin and its substitutes under low-energy collision-induced dissociation condition

✍ Scribed by Juan Peng; Lily Zu; Weihai Fang; Lingyun Huang; Yaru Wang; Dacheng He

• Book ID: 102380078
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 356 KB
• Volume: 45
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.1748

✦ Synopsis

Abstract

An understanding of the dissociation of penetratin is important for improving its metabolic stability and cargo‐delivery efficiency. In this study, we describe the selective cleavage of the K15–K16 amide bond of penetratin under the low‐energy collision‐induced dissociation condition in mass spectrometry. A variety of penetratin substitutes have been studied in which key basic amino acids have been substituted within the sequence. The calculated structure indicates that an α‐helix structure prevents the fragmentation of the central peptide domain and the side chain of lysine is involved in the proton translocation process. Copyright © 2010 John Wiley & Sons, Ltd.