Selective blockade of axonogenesis in cultured hippocampal neurons by the tyrosine phosphatase inhibitor orthovanadate
✍ Scribed by Mandell, James W. ;Banker, Gary A.
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 278 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
Protein tyrosine phosphorylation
reversible. An additional and unexpected effect of orhas been implicated in several aspects of neurite outthovanadate was the appearance of many binucleate growth regulation. To address specific roles in early neurons. Immunoblotting experiments using a phosneuronal morphogenesis, hippocampal neurons in culphotyrosine-specific antibody revealed an orthovanature were treated with the tyrosine phosphatase inhibdate-induced reversible hyperphosphorylation of sevitor orthovanadate. This treatment completely superal protein bands, especially of two at 115 and 125 pressed axon formation, yet enhanced formation of kD. These data suggest a potentially important role minor neurites. The inhibition of axonogenesis was for tyrosine phosphatases and their phosphoprotein dose dependent and occurred in parallel with a substrates in axonogenesis.