Selective Biochemical Modification of Functional Residues in Recombinant Human Macrophage Colony-Stimulating Factor β (rhM-CSF β): Identification by Mass Spectrometry †

Characterization of the folding pathway

Characterization of the folding pathway of recombinant human macrophage-colony stimulating-factor β (rhM-CSF β) by bis-cysteinyl modification and mass spectrometry

✍ H. Peter Happersberger; Janet Stapleton; Cynthia Cowgill; Michael O. Glocker 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 318 KB 👁 1 views

Melarsen oxide [p-(4,6-diamino-1,3,5-triazin-2-yl)aminophenylarsonous acid (MEL)], which selectively bridges spatially neighboring bis-cysteinyl residues in (reduced) proteins, was used to trap folding intermediates chemically during 1) time-dependent renaturation of recombinant human macrophage col