Selective and rapid solubilization of the microbial membrane enzyme aldehyde dehydrogenase

✍ Scribed by Dr. R. Hommel; H.-P. Kleber

• Book ID: 102393942
• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 256 KB
• Volume: 30
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620300418

✦ Synopsis

An improved solubilization procedure for the membrane-bound quinoprotein aldehyde dehydrogenase from Acetobacter rancens CCM 1774 was established. After the first solubilization of membrane enzymes by Brij 35 which provided important extraction of membrane proteins other than aldehyde dehydrogenase, the application of Trition X-I00 resulted in an almost 20-fold purification of quinoprotein aldehyde dehydrogenase. The optimal solubilization was closely connected with definite detergentlprotein ratios.