Selective absorption of anti-DNA antibodies and their idiotype-positive cells in vitro using an anti-idiotypic antibody-affinity column: Possible application to plasma exchange
✍ Scribed by Naoko Harata; Takeshi Sasaki; Shinobu Shibata; Junichi Kameoka; Yasuhiko Hirabayashi; Hirofumi Osaki; Eiichi Tamate; Kaoru Yoshinaga
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 492 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0733-2459
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✦ Synopsis
We examined the possibility of using affinity columns coupled with anti-idiotype (Id) antibodies to selectively remove nephritogenic anti-DNA antibodies in order to determine their possible application to therapeutic plasmapheresis. Monoclonal anti-Id antibodies termed D1E2 or 1F5 were directed to idotypes of human anti-single-stranded and anti-double-stranded DNA antibodies. The mixture of D1E2- and 1F5-coupled Sepharose absorbed 26 to 92% of human anti-DNA antibodies in sera. The affinity columns were also effective in removing anti-DNA idiotype-positive cells from the blood samples of patients, especially those with active lupus nephritis. Thus, an anti-idiotypic antibody-coupled affinity column could, in theory, serve as a tool for selective plasma exchange in the therapy of autoimmune disease.