Selection of β-lactamases and penicillin binding mutants from a library of phage displayed TEM-1 β-lactamase randomly mutated in the active site Ω-loop

In vitro selection for catalytic turnove

In vitro selection for catalytic turnover from a library of β-lactamase mutants and penicillin-binding proteins

✍ Bérangère Avalle; Sophie Vanwetswinkel; Jacques Fastrez 📂 Article 📅 1997 🏛 Elsevier Science 🌐 English ⚖ 346 KB

A library of mutants of the RTEM 13-1actamase displayed on phage was created; it contained penicillin binding proteins (PBPs) as well as a small fraction of active B-lactamases. The library was submitted to a selection process to extract the B-lactamases i.e. the enzymes that turnover efficiently. T