Selection of spontaneous mutants by inos
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Henry, Susan A. ;Donahue, Thomas F. ;Culbertson, Michael R.
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Article
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1975
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Springer
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English
โ 658 KB
A new method for the routine isolation of mutations of spontaneous origin in the yeast, Saccharomyces cerevisiae, is reported. The technique is based on the observation that inositol auxotrophs die when deprived of inositol. However, if macromolecular synthesis is inhibited, most of the cells surviv