## Abstract ## Background Because gene therapy of the future will primarily take an __in vivo__ approach, a number of problems associated with its current implementation exist. Currently, repeated delivery of a vector __in vivo__ is necessary to ensure adequate transfer of the therapeutic gene. Th
Selection of retroviral vector producing cell lines and gene transfer into hematopoietic cells
β Scribed by C. Bagnis; P. Mannoni
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 112 KB
- Volume
- 38
- Category
- Article
- ISSN
- 1269-3286
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Cord blood (CB) progenitor/stem cells (P/SC) are ideal targets for early gene therapy in individuals prenatally diagnosed with genetic disorders. Most retroviral transduction protocols were developed using adult peripheral blood stem cells (PBSC) and bone marrow (BM). Less is known about retroviral
We used a recombinant retrovirus as one of the potential vectors for human gene therapy to transfer a drug sensitivity gene into human lung cancer cells. The gene encoding the thymidine kinase (TK) of herpes simplex virus type 1 (HSVI) was used as the drug sensitivity gene. The antiherpes drugs acyc