Selection of reliable reference genes for quantitative real-time PCR in human T cells and neutrophils
โ Scribed by Carola Ledderose; Jens Heyn; Elisabeth Limbeck; Simone Kreth
- Book ID
- 115025121
- Publisher
- BioMed Central
- Year
- 2011
- Tongue
- English
- Weight
- 400 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1756-0500
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## Background Real-time quantitative PCR is a sensitive and very efficient technique to examine gene transcription patterns in preimplantation embryos, in order to gain information about embryo development and to optimize assisted reproductive technologies. Critical to the succesful application of
## Background Real-time quantitative PCR can be a very powerful and accurate technique to examine gene transcription patterns in different biological conditions. One of the critical steps in comparing transcription profiles is accurate normalisation. In most of the studies published on real-time PC