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Selection of plant heterokaryons from diverse origins by flow cytometry

✍ Scribed by N. Hammatt; A. Lister; N. W. Blackhall; J. Gartland; T. K. Ghose; D. M. Gilmour; J. B. Power; M. R. Davey; E. C. Cocking

Book ID
112521629
Publisher
Springer Vienna
Year
1990
Tongue
English
Weight
947 KB
Volume
154
Category
Article
ISSN
0033-183X

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Surface labelling of plant protoplasts w
Surface labelling of plant protoplasts with fluorochromes for discrimination of heterokaryons by microscopy and flow cytometry
✍ W.J.P. van Kesteren; M.J. Tempelaar πŸ“‚ Article πŸ“… 1993 πŸ› Elsevier Science 🌐 English βš– 591 KB

## Abstract Surface labelling of plant protoplasts was tested for use in mass fusion systems and heterokaryon detection. Parameters have been established for biotinylation and subsequent incubation with avidin‐coupled fluorochromes. The procedure is rapid (less than 3 hours) and does not affect via