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Selection of peptide ligands binding to the basolateral cell surface of proximal convoluted tubules

✍ Scribed by Audigé, Annette; Frick, Christoph; Frey, Felix J.; Mazzucchelli, Luca; Odermatt, Alex

Book ID
104474322
Publisher
Nature Publishing Group
Year
2002
Tongue
English
Weight
494 KB
Volume
61
Category
Article
ISSN
0085-2538

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## Abstract Using phage display technology, we have isolated 12‐mer peptide ligands that bind to human blood outgrowth endothelial cells (HBOEC). To avoid non‐specific binding we apply negative–positive selection approach by pre‐incubating the library with non‐HBOEC. The selected phage clones bind