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Selection of optimal reference genes for normalization in quantitative RT-PCR

✍ Scribed by Inna Chervoneva; Yanyan Li; Stephanie Schulz; Sean Croker; Chantell Wilson; Scott A Waldman; Terry Hyslop

Book ID
114999169
Publisher
BioMed Central
Year
2010
Tongue
English
Weight
717 KB
Volume
11
Category
Article
ISSN
1471-2105

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## Abstract Careful validation of reference genes used for the normalization of real‐time RT‐PCR data is required to obtain accurate results regarding gene expression. We evaluated the stability of seven commonly used reference genes in the cerebral cortex and hippocampus of rats 3 days following t