Selection of mutant Streptomyces strains with enhanced ability for acid precipitable polymeric lignin production
✍ Scribed by Dr. S. P. Antai; A. H. Iyo
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 330 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Abstract
Streptomyces viridosporus T7A spores were subjected to ultraviolet (UV) light mutagensis. Two mutant strains S. viridosporus T7A‐40 and T7A‐198 were isolated. These strains were grown on 5 g of sugar cane baggase lignocellulose and produced 36.0 mg and 41.4 mg of acid precipitable polymeric lignin (APPL) respectively as compared to 25.1 mg produced by the wild type after 8 weeks incubation at 37 °C. The quantities of APPL produced by the mutants represent 43.7% and 65.3% more APPLs than those produced by the wild type. Apart from the increase in APPL production, the observable effects of the ultraviolet light included; variation in spore pigmentation, induction of a brown diffusible pigment, production of hydrogen sulfide, loss of thermotolerance, lower cellulase activity and loss of inositol utilizing ability.