Selection of induced mutants with improved linolenic acid content in camelina

✍ Scribed by Vollmann, Johann ;Damboeck, Astrid ;Baumgartner, Sabine ;Ruckenbauer, Peter

• Publisher: John Wiley and Sons
• Year: 1997
• Weight: 498 KB
• Volume: 99
• Category: Article
• ISSN: 0931-5985
• DOI: 10.1002/lipi.19970991004

✦ Synopsis

Camelina (Camelina sariva (L.) Crtz.) is regarded as a potential crop producing a seed oil rich in linolenic acid (C18:3), which could be utilized for different oleochemical applications. Seeds of a camelina breeding line have been irradiated with gamma-rays in order to induce genetic variation in fatty acid composition. In the M2-generation, 8017 plants were subjected to a thiobarbituric acid test to identify mutants with increased linolenic acid content. Subsequently, M3lines were isolated, which showed significantly higher concentrations of linolenic acid (up to 40.8%) than the control (34-36%). Moreover, genotypes with an erucic acid content of less than 2% were also found in the mutant population. Different mutant lines can thus be combined in order to obtain transgressive segregants, which could give a further increase in linolenic acid content.

Selektion induzierter Mutanten mlt erhtihtem Linolenstluregehalt bei Leindotter. Leindotter (Carnelina saliva (L.) Crtz.) gilt wegen seines Gehalts an Linolensaure (C18:3) als potentielle Industrieolpflanze. Zur Induktion genetischer Variation im Fettsauremuster wurden Samen eines Zuchtstammes mit Gammastrahlen behandelt. Nach der Bestrahlung wurden in der M2-Generation 8017 Pflanzen mittels eines Thiobarbiturat-Farbtests untersucht, um Mutanten mit erhohtem Gehalt an Linolensaure zu identifizieren. In der M3-Generation wurden schliel3lich Linien mit einem