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Selection of human antibody fragments on the basis of stabilization of the variable domain in the presence of target antigens

โœ Scribed by Hideki Watanabe; Kouhei Tsumoto; Ryutaro Asano; Yoshiyuki Nishimiya; Izumi Kumagai


Book ID
117052089
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
164 KB
Volume
295
Category
Article
ISSN
0006-291X

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Contribution of variable domains to the
โœ Roxana M. Ionescu; Josef Vlasak; Colleen Price; Marc Kirchmeier ๐Ÿ“‚ Article ๐Ÿ“… 2008 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 354 KB

Temperature-induced unfolding of three humanized IgG1 monoclonal antibodies and their Fab and Fc fragments was monitored by differential scanning calorimetry at neutral pH. With some exceptions, the thermogram of the intact antibody presents two peaks and the transition with the larger experimental