Selection of gamma-ray mutants from a strain ofHumicola lutea72, producing acid proteases

✍ Scribed by I. Grigorov; P. Aleksieva; A. Djerova; P. Sheremetska; B. Tchorbanov

• Book ID: 104787653
• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 226 KB
• Volume: 17
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00499503

✦ Synopsis

Gamma-ray exposure was used as a mutagenic agent for mutant induction from a strain of Humicola lutea 72 producing acid proteases. The second stage of irradiation resulted in a double active mutant (H. lutea 120-8). Further treatment gave rise to a triple active mutant (H. lutea 120-5) with a maximal proteolytic activity of 1,800 ~g tyrosine liberated from 2% casein/m1-1 culture filtrate/min -1 at pH 3,0 and 40 ~ C. A change in the composition of proteolytic enzymes caused by gamma-ray exposure was established, using chromatography on DEAE-cellulose.