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Selection methods for high-producing mammalian cell lines

✍ Scribed by Susan M. Browne; Mohamed Al-Rubeai

Book ID
113932085
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
545 KB
Volume
25
Category
Article
ISSN
0167-7799

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## Abstract **BACKGROUND:** Human embryonic kidney‐293 (HEK‐293) cells are commonly used as a transient expression host but their application in stable therapeutic protein production is limited. This is presumably due to the absence of a suitable amplifiable expression system and hence limited prot