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Segmentation of Fluorescence Microscopy Images for Quantitative Analysis of Cell Nuclear Architecture

โœ Scribed by Russell, Richard A.; Adams, Niall M.; Stephens, David A.; Batty, Elizabeth; Jensen, Kirsten; Freemont, Paul S.


Book ID
119205001
Publisher
Biophysical Society
Year
2009
Tongue
English
Weight
758 KB
Volume
96
Category
Article
ISSN
0006-3495

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## Abstract Standard fluorescence microscopy approaches rely on measurements at single excitation and emission bands to identify specific fluorophores and the setting of thresholds to quantify fluorophore intensity. This is often insufficient to reliably resolve and quantify fluorescent labels in t