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Segmental isotope labeling of proteins for NMR structural study using a protein S tag for higher expression and solubility

โœ Scribed by Hiroshi Kobayashi; G. V. T. Swapna; Kuen-Phon Wu; Yuliya Afinogenova; Kenith Conover; Binchen Mao; Gaetano T. Montelione; Masayori Inouye


Book ID
111912805
Publisher
Springer Netherlands
Year
2012
Tongue
English
Weight
598 KB
Volume
52
Category
Article
ISSN
0925-2738

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Expression and purification of an isotop
โœ Michael Piazza; Yay Duangkham; Donald E. Spratt; Thorsten Dieckmann; J. Guy Guil ๐Ÿ“‚ Article ๐Ÿ“… 2011 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 250 KB

Nuclear magnetic resonance (NMR) spectroscopy is an efficient method for studying the dynamics and structures of proteinโ€protein and proteinโ€peptide complexes. Calmodulin (CaM), a ubiquitous Ca^2+^โ€sensing protein, is able to bind and regulate various intracellular proteins, including the nitric oxi