Sedimentation-equilibrium studies of the polysaccharide components of Pseudomonas aeruginosa

✍ Scribed by Derek Horton; David A. Riley; Poul M. T. Hansen

• Book ID: 102765084
• Publisher: Wiley (John Wiley & Sons)
• Year: 1980
• Tongue: English
• Weight: 725 KB
• Volume: 19
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1980.360191009

✦ Synopsis

Abstract

Mild acid hydrolysis of lipopolysaccharide antigens from seven different serotype strains antigen immunotypes nos. 1–7 [in the classification of Fisher, M. W., Devlin, H. B. & Gnabasik, F. J. (1969) J. Bacteriol. 98, 835–836] of Pseudomonas aeruginosa gave polysaccharide components of high molecular weight, which were isolated by gel filtration and dialysis. These components were examined by ultracentrifugation at equilibrium with the Rayleigh interferometric optical system. The partial specific volumes were calculated from densities obtained by using a mechanical oscillator. The average molecular weights (M~n~, M~w~, and M~z~) were calculated and compared to evaluate the polydispersity of the polysaccharides. The nonideality was investigated by varying the rotor speed, the height of the solution column, and the concentrations of the polysaccharide fractions. The molar masses were found to range from 14,000 for the polysaccharide from immunotype two to 24,000 for that from immunotype one, when extrapolated to zero rotor speed and solution column height.