Secretory granules and nissl substance in fresh supraoptic neurones of the rabbit
✍ Scribed by Palay, Sanford L. ;Wissig, Steven L.
- Publisher
- John Wiley and Sons
- Year
- 1953
- Tongue
- English
- Weight
- 631 KB
- Volume
- 116
- Category
- Article
- ISSN
- 0003-276X
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✦ Synopsis
Haven, Connec ticzit FOUR FIGURES
Application of the chrome-alum hematoxylin stain to the hypothalamic neurones of vertebrates (Bargmann, '49) has revealed a new type of neurosecretory material in the form of extremely fine granules close to the limit of resolution of the light microscope. Smith ('51) has established the identity of these granules with the neurosecretory material disclosed by other staining methods. Considerable interest attaches to the question of whether these granules exist as such in the living neurone or perhaps result from precipitation during fixation of a soluble rieurosecretory substance diffused through the cytoplasm of the neurones. This paper reports an investigation of fresh, living, supraoptic neurones with the phase-contrast microscope. The observations indicate that secretory granules are demonstrable in these nerve cells and their processes without the intervention of fixatives and stains.
The additional observation of distinct linear striations in the Nissl substance of these neurones is reported and is interpreted as the ifi vizio manifestation of the endoplasmic reticulum disclosed by electron microscopy of fixed and sectioned