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Secretory Expression of Insulin Precursor in Pichia pastoris and Simple Procedure for Producing Recombinant Human Insulin

โœ Scribed by Xie, T.; Liu, Q.; Xie, F.; Liu, H.; Zhang, Y.


Book ID
126846706
Publisher
Taylor and Francis Group
Year
2008
Tongue
English
Weight
217 KB
Volume
38
Category
Article
ISSN
1082-6068

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Human insulin from a precursor overexpre
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The methylotrophic yeast Pichia pastoris, which proved successful in producing many heterologous proteins, was used to express an insulin precursor. A transformant with a high copy number of the gene integrated into the chromosome was obtained by the dot-blotting method. In high-density fermentation