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Secretion of proteins with dimerization capacity by the haemolysin type I transport system of Escherichia coli

✍ Scribed by Sofía Fraile; Amalia Muñoz; Víctor De Lorenzo; Luis Angel Fernández


Book ID
110832133
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
292 KB
Volume
53
Category
Article
ISSN
0950-382X

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We studied the efficiency of the pHly152-derived haemolysin transport system using PhoA-HlyA fusion proteins and different constructs which provide HlyB/HlyD in trans. The optimal C-terminal HlyA signal consists of the last 60 amino acids. Longer stretches of HlyA do not improve the transport effici