𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Secretion of newly synthesized proteins into the extracellular fluid of the rabbit hippocampus

✍ Scribed by B. Nyström; J.-O. Karlsson; Professor A. Hamberger


Book ID
102384462
Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
413 KB
Volume
21
Category
Article
ISSN
0360-4012

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✦ Synopsis


Extracellular proteins were collected continuously from the hippocampus of the rabbit by slow perfusion of a protein-permeable thin tubing. After pulse labelling with radioactive amino acids via the tubing a rapid appearance of labelled proteins was seen in the extracellular fluid. The half-life of the proteins in the extracellular space varied between 15 min and 2 h. Protein fractions were separated with gel electrophoresis or HPLC. The extracellular fraction differed distinctly from intracellular proteins with respect to the labelling pattern.


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