Secretion of gelatinases and tissue inhibitors of metalloproteinases by human lung cancer cell lines and revertant cell lines: Not an invariant correlation with metastasis

✍ Scribed by Stanley Zucker; Rita M. Lysik; Masood Malik; Brett A. Bauer; J. Caamano; Andres J. P. Klein-Szanto

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: French
• Weight: 729 KB
• Volume: 52
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910520307

✦ Synopsis

Numerous studies have reported a correlation between production of 72-kDa (MMP-2) and 92-kDa (MMP-9) type-IV collagenases/gelatinases and the metastatic potential of cancer cells. An abrogating effect of tissue inhibitors of metalloproteinases (TIMP-I and TIMP-2) on metastases has also been noted.

In this report we have used sensitive enzyme-linked immunoassays to measure MMP-2, MMP-9, TIMP-I and TIMP-2 levels in eight human lung-cancer cell lines which were characterized for biological behavior in nude mice. We demonstrated that the C a b 6 and A549 cell lines with the highest metastatic, invasive and tumorigenic potential secreted the highest levels of MMP-2. MMP-9 and TIMP-I secretions were comparatively low in all cell lines. TIMP-2 secretion, which exceeded MMP-2 secretion for all cell lines, did not correlate with metastatic potential. To further explore these correlations, the metastatic C a b 6 cell line was transfected with a K-rev-I cDNA expression construct.