Secretion of exo- and endo-β-N-acetylglucosaminidases by insect cell lines
✍ Scribed by C.Dziadik Turner; Daizo Koga; Karl J. Kramer
- Book ID
- 113167956
- Publisher
- Elsevier Science
- Year
- 1981
- Weight
- 400 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0020-1790
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We have developed an assay system for endo--Nacetylglucosaminidase and glycoamidase (PNGase), using Eu 3؉ -labeled Man 9 GlcNAc 2 glycopeptides as substrates in combination with lectin capture. Two glycopeptides of different peptide lengths, derived from soybean agglutinin, were labeled with Eu 3؉
## Abstract __N‐__acetylneuraminic acid (Neu5Ac) and __N‐__glycolylneuraminic acid (Neu5Gc) are the dominant sialic acids (Sia) in mammals usually found in the non‐reducing terminal of oligosaccharide side chains in glycoproteins and glycolipids. Their expression and distribution pattern have been