Secretion in tissue cultures. III. Tonicity of fluid in chick mesonephric cysts

✍ Scribed by Keosian, John

• Publisher: Wiley (John Wiley & Sons)
• Year: 1938
• Tongue: English
• Weight: 785 KB
• Volume: 12
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030120103

✦ Synopsis

Fragments of chick mesonephros grown in tissue culturc remain alive for several days and exhibit undoubted evidence of functional activity (Chambers and Kempton, '33). Many of the isolated segments of proximal tubule close at their ends and become distended into cysts from the accumulation of fluid within their lumina (figs. 1 arid 2). Chambers and Kempton have shown that the distension of these segments is accompanied by an active secretory process. Confirmatory evidence that this is an active secretion is furnished by the paper of Chambers, Beck and Belkin ( '35). Maximum distension of the cysts is reached in about 48 hours (fig. 2 ) . The cysts vary in size depending on the original size of the tubule from which each was respectively formed. The average size of those large enough to permit withdrawal of fluid was 0.0006

The problem suggested itself of determining the differencc in tonicity between the fluid accumulated in the cysts and that surrounding them in the culture medium. The withdrawal of the fluids was accomplished by means of micropipettes controlled by a micromanipulator. The results of cc.