The synthesis of a proteolytically unstable protein, originally designed for periplasmic export in recombinant Escherichia coli BL21(DE3), a strain naturally deficient for the ATP-dependent protease Lon (or La) and the outer membrane protease OmpT, is associated with a severe growth inhibition. This
✦ LIBER ✦
Secretion-dependent proteolysis of recombinant proteins is associated with inhibition of cell growth in Escherichia coli
✍ Scribed by Elisenda Viaplana; Xavier Rebordosa; Jaume Pi–ol; Antonio Villaverde
- Book ID
- 110369502
- Publisher
- Springer Netherlands
- Year
- 1997
- Tongue
- English
- Weight
- 519 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0141-5492
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